Coding

Part:BBa_K1088004:Design

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-06)


IspG (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 343


Design Notes

We designed primers to isolate IspG directly from the genome by colony PCR. The primers included an overhang containing the biobrick prefix and suffix


Source

Isolated from E. coli MG1655

References